Journal
/
/
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
DOI:

09:59 min

March 16, 2017

, ,

Chapitres

  • 00:05Titre
  • 00:39Live Cell Imaging by TIRFM
  • 02:48Imaging SEP to Determine Subcellular Localization
  • 04:39Imaging Single Vesicle Insertion Events in Live Cells
  • 05:44Image Analysis and Data Processing
  • 08:17Results: Monitoring the Localization, Expression, and Trafficking of Membrane Proteins
  • 09:30Conclusion

Summary

Traduction automatique

Labeling the extracellular domain of a membrane protein with a pH sensitive fluorophore, superecliptic pHluorin (SEP), allows subcellular localization, expression, and trafficking to be determined. Imaging SEP-labeled proteins with total internal reflection fluorescence microscopy (TIRFM) enables the quantification of protein levels in the peripheral ER and plasma membrane.

Vidéos Connexes

Read Article