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Metoder til ekstraktion af endosymbionter fra Whitefly

Article DOI: 10.3791/55809-v • 07:28 min • June 19th, 2017
June 19th, 2017 •
1, 1, 1, 1, 1, 1
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences, Zhejiang University

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Her præsenterer vi en protokol til isolering af endosymbionter fra whitefly Bemisia tabaci gennem dissektion og filtrering. Efter amplifikation er DNA-prøverne egnede til efterfølgende sekventering og undersøgelse af gensidigheden mellem endosymbioner og whitefly.

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Molekylærbiologi udgave 124, Endosymbiont genom-sekventering lokalisering oprensning whitefly
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