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Biochemistry

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Eksempel forberedelse til masse massespektrometri-baserte identifikasjon av RNA-bindende områder

Article DOI: 10.3791/56004-v • 10:52 min • September 28th, 2017
September 28th, 2017 •
1,4, 1,2, 1,3, 1,3, 1,2
1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

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Vi beskriver en protokoll for å identifisere RNA-bindende proteiner og tilordne sine RNA-bindende regioner i levende celler ved hjelp av UV-mediert photocrosslinking og massespektrometri.

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Biokjemi problemet 127 Protein-RNA interaksjoner RNA-bindende domene RNA-bindende regionen massespektrometri noncoding RNAs UV crosslinking
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