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Måling af mRNA niveauer Over tid under gær S. cerevisiae hypoksiske svar

Article DOI: 10.3791/56226-v • 09:45 min • August 10th, 2017
August 10th, 2017 •
1, 2, 2, 2
1Department of Molecular Biology, Rowan School of Osteopathic Medicine, 2Department of Biological Sciences, Rowan University

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Vi præsenterer her, en protokol, ved hjælp af RNA-seq for at overvåge mRNA niveauer over tid under det hypoksiske svar af S. cerevisiae celler. Denne metode kan tilpasses til at analysere genekspression under nogen cellulære reaktion.

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