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Bioengineering

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JoVE Journal Bioengineering
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Correlative lys elektronmikroskop (CLEM) for sporing og Imaging Viral Protein forbundet strukturer i Cryo-immobilisert celler

Article DOI: 10.3791/58154-v • 09:18 min • September 7th, 2018
September 7th, 2018 •
1, 2, 1, 3, 1, 1, 2,4, 2
1European Molecular Biology Laboratory, 2Department of Infectious Diseases, Molecular Virology, Heidelberg University, 3Central Facility for Electron Microscopy, Ulm University, 4Heidelberg Partner Site, German Center for Infection Research

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En correlative lys elektronmikroskop (CLEM) metode brukes bilde virus-indusert intracellulære strukturer via elektronmikroskop (EM) i cellene som er tidligere valgt av * lys (LM). LM og EM kombineres som en hybrid tenkelig tilnærming til å oppnå en integrert visning av virus-vert interaksjoner.

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Bioteknologi problemet 139 høytrykk frysing fryse substitusjon vitrifikasjon målrettet trimming overføring elektronmikroskop safir plater cellekultur virus infeksjon virus-vert interaksjoner ultrastructure forårsaket av virus mobilnettet endringer.
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