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Bioengineering
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Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

Article DOI: 10.3791/2909-v • 10:00 min • June 5th, 2011
June 5th, 2011 •
1, 1
1Department of Molecular and Cellular Biology, University of California Davis

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We demonstrate the fabrication of a low-cost cryogenic stage designed to fit most reflected light microscopes. This lab-built cryogenic stage enables efficient and reliable correlative imaging between cryo-light and cryo-electron microscopy.

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Cryo-light Microscopy Cryo-electron Microscopy Correlated Microscopy Cellular Dynamics Ultrastructure Cryogenic Stage Cryo-LM Cryo-EM Sample Preservation Vitrification Process Correlative Imaging Inexpensive Cryogenic Stage Reflected Light Microscopes Long Working Distance Air Objectives Carbon Coated Cryo-EM Grids Multi-technique Imaging
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