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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifiering av mus och Human antikropp repertoarer av nästa generations sekvensering
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Identifiering av mus och Human antikropp repertoarer av nästa generations sekvensering

Article DOI: 10.3791/58804-v • 08:51 min • March 15th, 2019
March 15th, 2019 •
1, 2, 3, 1, 4,5, 4, 4, 6, 7,8, 4,5, 9, 2, 9,10
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Center for Influenza Virus Research, National Institute of Infectious Diseases, 3School of Science and Technology, Kwansei Gakuin University, 4Department of Pathology, National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, 9Department of Immunology, National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences, University of Tsukuba

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Här beskriver vi protokoll för analys och visualisering av strukturen och konstitutionen av hela antikropp repertoarer. Detta innebär förvärvet av stora sekvenser av antikropp RNA med hjälp av nästa generations sekvensering.

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Immunologi och infektion fråga 145 antikropp repertoar nästa generations sekvensering NGS RNA-seq immunsvar Antigen erkännande V-D-J rekombination immunglobulin
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