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Isolamento e cultura de células endoteliais aórticas primárias de suínos em miniatura

Article DOI: 10.3791/59673-v • 06:38 min • August 14th, 2019
August 14th, 2019 •
1,2,3, 4, 5, 2, 6, 3, 3, 3, 1, 2, 1,2,3
1Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, 3Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 4Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 5Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, 6People's Hospital of Longhua

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Um método enzimático eficaz para o isolamento de células endoteliais aórticas primárias porcinas (pAECs) de suínos em miniatura é descrito. Os pAECs preliminares isolados podem ser usados para investigar a resposta imune e da coagulação no xenotransplante.

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Biologia edição 150 células CD31+ cultura celular porco em miniatura células endoteliais aórticas porcinas isolamento de células primárias xenotransplante
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