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Derivazione della sfera di tumore e trattamento da cellule tumorali primarie isolate da Rhabdomyosarcomas del topo

Article DOI: 10.3791/59897-v • 09:21 min • September 13th, 2019
September 13th, 2019 •
1,2,3, 2
1Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery, University California San Diego

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Questo protocollo descrive un metodo riproducibile per l'isolamento delle cellule primarie del thabdomyosarcoma del topo, la formazione e il trattamento della tumorsfera e il trapianto di allotrapianto a partire dalle colture di cellule di tumori.

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Genetica Problema 151 tumorsferai rhabdomyosarcoma muscolo scheletrico cellule primarie trattamento proteico ricombinante trasfezione plasmide
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