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JoVE Journal Medicine
Flow Cytometrie Analyse van immune cell subsets binnen de Murine Milt, Beenmerg, lymfeklieren en synoviale weefsel in een artrose model
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Flow Cytometrie Analyse van immune cell subsets binnen de Murine Milt, Beenmerg, lymfeklieren en synoviale weefsel in een artrose model

Article DOI: 10.3791/61008-v • 12:23 min • April 24th, 2020
April 24th, 2020 •
1,2, 1,3, 1, 1, 1, 1
1Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital, University of Sydney, 2HTRG - Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery, Heidelberg University Hospital, 3Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University

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Hier beschrijven we een gedetailleerd en reproduceerbaar stroomcytometrieprotocol om monocyten/macrofaag en T-celsubsets te identificeren met behulp van zowel extra- als intracellulaire kleuringstesten in de murine milt, beenmerg, lymfeklieren en synovaal weefsel, gebruikmakend van een gevestigd chirurgisch model van murineartrose.

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Geneeskunde Artrose immunologie flow cytometrie lymfocytensubsets T-cellen monocyten macrofagen orthopedie posttraumatische artrose
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