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Bioengineering

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In Vitro Model af Human Kutan hypertrofisk ardannelse ved hjælp af makromolekylære fortrængning

Article DOI: 10.3791/61037-v • 08:20 min • May 1st, 2020
May 1st, 2020 •
1, 1, 1, 1, 1,2, 1, 1,3, 1
1Skin Research Institute of Singapore, Agency for Science, Technology and Research (A*STAR), 2School of Chemical and Life Sciences, Singapore Polytechnic, 3Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR)

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Denne protokol beskriver brugen af makromolekylær fortrængning til at skabe en in vitro human hypertrofisk arvæv model, der ligner in vivo betingelser. Når de dyrkes i et overfyldt makromolekylært miljø, udviser hudfibroblaster fænotyper, biokemi, fysiologi og funktionelle egenskaber, der ligner arvæv.

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Bioengineering hypertrofisk ar fibroblaster makromolekylær fortrængning kollagen ekstracellulær matrix tæthedgradient medium polyvinylpyrrolidon
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