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Developmental Biology

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JoVE Journal Developmental Biology
Differentiering och karakterisering av neurala stamceller och nervceller från mus embryonala stamceller
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Differentiering och karakterisering av neurala stamceller och nervceller från mus embryonala stamceller

Article DOI: 10.3791/61446-v • 08:47 min • May 15th, 2020
May 15th, 2020 •
1, 1, 1, 1
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine, Penn State Hershey Cancer Institute

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Vi beskriver förfarandet för in vitro differentiering av mus embryonala stamceller i neuronala celler med hjälp av hängande dropp metod. Dessutom utför vi en omfattande fenotypisk analys genom RT-qPCR, immunofluorescens, RNA-seq och flöde cytometri.

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Utvecklingsbiologi nummer 159 Musembry embryonala stamceller embryoida kroppar neural stamcell nervceller differentiering hängande droppe E14
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