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Bioengineering

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JoVE Journal Bioengineering
Manipulation av single neurala stamceller och nervceller i hjärnan skivor med hjälp av Robotic Microinjection
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Manipulation av single neurala stamceller och nervceller i hjärnan skivor med hjälp av Robotic Microinjection

Article DOI: 10.3791/61599-v • 11:40 min • January 21st, 2021
January 21st, 2021 •
*1,2, *3, 3, 3,4, 1,5,6
1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota
* These authors contributed equally

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Detta protokoll visar användningen av en robotplattform för microinjection i enstaka neurala stamceller och nervceller i hjärnan skivor. Denna teknik är mångsidig och erbjuder en metod för att spåra celler i vävnad med hög rumslig upplösning.

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Bioengineering hjärnans utveckling stamceller härstamning spårning robotik neuron mikroinjektion organotypisk vävnad skiva encellig manipulation
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