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Agrobacterium-Mediato Trasformazione Genetica, Produzione Transgenica, e la sua applicazione per lo studio dello sviluppo riproduttivo maschile nel riso

Article DOI: 10.3791/61665-v • 07:43 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1, 1, 3, 1,3
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide
* These authors contributed equally

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Questo lavoro descrive l'uso della tecnologia di editing del genoma CRISPR-Cas9 per knockout gene endogeno OsABCG15 seguita da un protocollo di trasformazione mediato Agrobacterium modificatoper produrre una linea stabile maschio-sterile nel riso.

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Questo mese in JoVE Problema 164 riso CRISPR-Cas9 Agrobacterium callo coltura dei tessuti genotipo fenotipo anther polline
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