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Biochemistry

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JoVE Journal Biochemistry
Single cell analyse van transcriptioneel actieve allelen door single molecule FISH
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Single cell analyse van transcriptioneel actieve allelen door single molecule FISH

Article DOI: 10.3791/61680-v • 06:26 min • September 20th, 2020
September 20th, 2020 •
1,2, 1,3, 1,2, 1,2, 1,2,3,4
1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology, Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, 4Department of Pharmacology and Chemical Biology, Baylor College of Medicine

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Single molecule RNA fluorescentie in situ hybridisatie (smFISH) is een methode om niveaus en lokalisatie van specifieke RNA's op het niveau van één cel nauwkeurig te kwantificeren. Hier rapporteren we onze gevalideerde laboratoriumprotocollen voor natte-bench verwerking, beeldvorming en beeldanalyse voor eencellige kwantificering van specifieke RNA's.

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Biochemie Nummer 163 Single molecule FISH beeldvorming beeldanalyse gentranscriptie
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