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Immunology and Infection

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JoVE Journal Immunology and Infection
En DNA/Ki67-baserad flödescytometrianalys för cellcykelanalys av antigenspecifika CD8 T-celler hos vaccinerade möss
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En DNA/Ki67-baserad flödescytometrianalys för cellcykelanalys av antigenspecifika CD8 T-celler hos vaccinerade möss

Article DOI: 10.3791/61867-v • 09:17 min • January 5th, 2021
January 5th, 2021 •
*1,2, *1,2, 3, 4, 5, 5, 2,6, 1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed
* These authors contributed equally

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Klonisk expansion är en viktig egenskap hos antigenspecifika T-cellssvar. Cellcykeln av antigen-svarande T-celler har dock dåligt undersökts, delvis på grund av tekniska begränsningar. Vi beskriver en flöde cytometric metod för att analysera clonally expandera antigen-specifika CD8 T celler i mjälte och lymfkörtlar av vaccinerade möss.

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Immunologi och infektion nummer 167 antigenspecifika CD8 T-celler cellcykel Ki67 DNA-färgämne flödescytometri mjälte lymfkörtlar mus
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