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Immunology and Infection

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Visualisierung von SARS-CoV-2 mit Immuno-RNA-Fluoreszenz in situ Hybridisierung

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Hier beschreiben wir eine einfache Methode, die RNA-Fluoreszenz-In-situ-Hybridisierung (RNA-FISH) mit Immunfluoreszenz kombiniert, um schweres akutes Atemwegssyndrom Coronavirus 2 (SARS-CoV-2) RNA zu visualisieren. Dieses Protokoll kann das Verständnis der molekularen Eigenschaften von SARS-CoV-2 RNA-Host-Wechselwirkungen auf einzelliger Ebene erhöhen.

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Immunologie und Infektion Ausgabe 166 SARS-CoV-2 RNA-FISH Hybridisierungskettenreaktion Immunfluoreszenz Human Airway Epithelium (HAE) Permeabilisation Konfokalmikroskopie
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