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Immunofenotyping og cellesortering av menneskelige MKs fra humane primærkilder eller differensiert in vitro fra hematopoietiske forfedre
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Immunofenotyping og cellesortering av menneskelige MKs fra humane primærkilder eller differensiert in vitro fra hematopoietiske forfedre

Article DOI: 10.3791/62569-v • 14:30 min • August 7th, 2021
August 7th, 2021 •
1, 1, 2, 1,3, 1, 4, 5, 1,6
1Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 2Flow Cytometry and Cell Sorting Platform (ISPA), 3Clinical Diagnosis Laboratory - Dept. of Hematology, Hospital Universitario Central de Asturias (HUCA), 4Department of Hematology, Hospital Universitario Severo Ochoa (HUSO), 5Department of Hematology, Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), 6Dept. of Medicine, University of Oviedo

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Her presenterer vi en immunofenotypingsstrategi for karakterisering av megakaryocyttdifferensiering, og viser hvordan denne strategien tillater sortering av megakaryocytter på forskjellige stadier med en fluorescensaktivert cellesortering. Metodikken kan brukes på humant primærvev, men også på megakaryocytter generert i kultur in vitro.

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Immunologi og infeksjon utgave 174
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