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Immunology and Infection

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JoVE Journal Immunology and Infection
Et automatiseret kultursystem til brug i præklinisk test af værtsstyrede terapier til tuberkulose
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Et automatiseret kultursystem til brug i præklinisk test af værtsstyrede terapier til tuberkulose

Article DOI: 10.3791/62838-v • 09:34 min • August 16th, 2021
August 16th, 2021 •
1, 1,2, 2, 2,3,4, 1, 1
1Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin, The University of Dublin, 2School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), 3SFI Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, 4SFI Centre for Research in Medical Devices (CURAM), RCSI, Dublin and National University of Ireland

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Hurtig og effektiv kvantificering af intracellulær M. tuberkulosevækst er afgørende for at forfølge forbedrede terapier mod tuberkulose (TB). Denne protokol beskriver et bouillonbaseret kolorimetrisk detektionsassay ved hjælp af et automatiseret væskekultursystem til kvantificering af Mtb-vækst i makrofager behandlet med kandidatværtsstyrede terapier.

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Immunologi og infektion udgave 174
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