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JoVE Journal Neuroscience
Generering av menneskelige motorenheter med funksjonelle nevromuskulære veikryss i mikrofluidiske enheter
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Generering av menneskelige motorenheter med funksjonelle nevromuskulære veikryss i mikrofluidiske enheter

Article DOI: 10.3791/62959-v • 10:48 min • September 7th, 2021
September 7th, 2021 •
1,2, 1,2, 1,2, 1,2, 3,4, 3,4, 5, 3,4, 1,2, 1,2, 5, 1,2,6, 1,2
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute, KU Leuven - University of Leuven, 2VIB Center for Brain & Disease Research, Laboratory of Neurobiology, , 3VIB Center for Brain & Disease Research, Research Group Molecular Neurobiology, , 4VIB Bio Imaging Core, KU Leuven - University of Leuven, 5Department of Development and Regeneration, Stem Cell and Developmental Biology, KU Leuven - University of Leuven, 6Department of Neurology, University Hospitals Leuven

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Vi beskriver en metode for å generere menneskelige motorenheter i kommersielt tilgjengelige mikrofluidiske enheter ved å kokulturere menneskeskapte pluripotente stamcelleavledede motoriske nevroner med humane primære mesoangioblast-avledede myotuber som resulterer i dannelse av funksjonelt aktive nevromuskulære veikryss.

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