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Developmental Biology

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JoVE Journal Developmental Biology
Generering av Airway Epitelial Cell Air-Liquid Interface Kulturer fra Human Pluripotent Stamceller
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Generering av Airway Epitelial Cell Air-Liquid Interface Kulturer fra Human Pluripotent Stamceller

Article DOI: 10.3791/63882-v • 10:46 min • June 14th, 2022
June 14th, 2022 •
1,2, 2, 3, 2, 2, 3, 1,2, 1,2
1Pulmonary Center, Boston University School of Medicine, 2Center for Regenerative Medicine, Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center

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Nylige fremskritt i menneskeskapte pluripotente stamcelledifferensieringsprotokoller tillater trinnvis avledning av organspesifikke celletyper. Her gir vi detaljerte trinn for vedlikehold og utvidelse av iPSC-avledede luftveisbasalceller og deres differensiering i et mucociliary epitel i luft-væske grensesnittkulturer.

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Utviklingsbiologi utgave 184
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