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Cancer Research

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JoVE Journal Cancer Research
Visualisera DNA-skador reparera proteiner i patient-derived äggstockscancer organoider via immunofluorescensanalyser
Plating Intact Patient-Derived Organoids and Setting Up Imaging Parameters for Multiplexed Live-Cell Imaging
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Plating Intact Patient-Derived Organoids and Setting Up Imaging Parameters for Multiplexed Live-Cell Imaging
A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)
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Visualisera DNA-skador reparera proteiner i patient-derived äggstockscancer organoider via immunofluorescensanalyser

Article DOI: 10.3791/64881-v • 04:07 min • February 24th, 2023
February 24th, 2023 •
1, 1, 1, 1, 1, 1,2, 1, 1
1Washington University in St. Louis, 2University of California San Francisco

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Detta protokoll beskriver metoder för att utvärdera DNA-skadereparationsproteiner i patient-derived ovarial cancer organoids. Här ingår omfattande pläterings- och färgningsmetoder samt detaljerade, objektiva kvantifieringsförfaranden.

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Cancerforskning nummer 192
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