Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
13,015 Views
•
08:51 min
•
May 27, 2008
DOI:
Summary
Automatically generated
We used synchrotron X-ray tomography at the European Synchrotron Radiation Facility (ESRF) to non-invasively produce 3D tomographic datasets with a pixel-resolution of 0.7µm. Using volume rendering software, this allows the reconstruction of internal structures in their natural state without the artefacts produced by histological sectioning.
Explore More Videos
Non-invasive3D VisualizationSub-micron ResolutionSynchrotron-X-ray-tomographyMicro-arthropodsInternal OrganizationSmall SizeHard CuticleClassical HistologyNon-destructive MethodSynchrotron X-ray TomographyEuropean Synchrotron Radiation Facility (ESRF)Grenoble (France)3D Tomographic DatasetsPixel-resolutionVolume Rendering SoftwareQuantitative MorphologyLandmarksAnimated MoviesHidden Body PartsComplete Organ Systems
Read Article
Cite this Article
Heethoff, M., Helfen, L., Cloetens, P. Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography. J. Vis. Exp. (15), e737, doi:10.3791/737 (2008).
Copy