Tumor Stroma Interaction
Tumor Stroma Interaction - Related Videos
07:20
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
0 Views •
Cited by 22 •
2020
A novel three-dimensional spheroid model based on the heterotypic interaction of tumor cells and stromal fibroblasts is established. Here, we present coculture of tumor cells and stromal fibroblasts, time-lapse imaging, and confocal microscopy to visualize the formation of spheroids. This three-dimensional model offers a pertinent platform to study tumor-stroma interactions and test cancer therapeutics.
08:40
A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
0 Views •
Cited by 24 •
2020
This protocol presents a 3D biomimetic model with accompanying fibrotic stromal compartment. Prepared with physiologically relevant hydrogels in ratios mimicking the bio-physical properties of the stromal extracellular matrix, an active mediator of cellular interactions, tumor growth and metastasis.
08:39
Three-dimensional Co-culture Model for Tumor-stromal Interaction
0 Views •
Cited by 14 •
2015
Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.
08:26
MAME Models for 4D Live-cell Imaging of Tumor: Microenvironment Interactions that Impact Malignant Progression
0 Views •
Cited by 43 •
2012
We have developed 3D coculture models for live-cell imaging in real-time of interactions among breast tumor cells and other cells in their microenvironment that impact progression to an invasive phenotype. These models can serve as preclinical screens for drugs to target paracrine-induced proteolytic, chemokine/cytokine and kinase pathways implicated in invasiveness.
01:24
Protein Transport to the Stroma
0 Views •
2023
Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function. Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
FAQs
Signals move between cancer cells and surrounding fibroblasts, immune cells, blood vessels, and extracellular matrix through cytokines, growth factors, and direct cell contact. These exchanges can alter the behavior of both malignant and nonmalignant components rather than affecting cancer cells alone. In engineered models, controlling which components are present helps researchers examine specific communication pathways and their consequences.
Extracellular matrix remodeling changes the surrounding tissue and can support several tumor-promoting processes. Within the tumor microenvironment, matrix changes are linked with invasion and can contribute to conditions that support angiogenesis or suppress immune activity. Recreating this remodeling in a bioengineered system allows researchers to investigate how the physical and signaling environment influences tumor progression.
Stromal communication can change how a tumor responds to therapy because cancer cells receive signals from fibroblasts, immune cells, blood vessels, and the extracellular matrix. A treatment may therefore produce different effects in cancer cells alone than in a more complete microenvironment. Models that include these interactions can help evaluate drug responses and combination treatments under controlled conditions.
These stromal components provide distinct sources of signals and interactions around cancer cells. Fibroblasts, immune cells, and blood vessels can participate in communication through cytokines, growth factors, or cell contact, while their combined activity may support angiogenesis or reduce immune activity. Studying them together helps reveal how multiple microenvironmental influences shape tumor behavior.
Researchers recreate tumor-stroma relationships using three-dimensional cultures, organoids, biomaterial scaffolds, and microfluidic systems. These platforms provide controlled settings in which cancer cells can be studied alongside selected stromal components and extracellular matrix features. Comparing such engineered environments can clarify how specific interactions affect tumor progression, invasion, and responses to treatment without relying only on simplified cancer-cell systems.
The choice depends on the interaction researchers want to recreate and control. Three-dimensional cultures and organoids can represent tumor organization, while biomaterial scaffolds and microfluidic systems provide engineered environments for examining relationships within a defined setting. Together, these approaches support systematic investigation of cancer-stroma communication and allow researchers to test how altered conditions affect experimental outcomes.
These models can show how communication with surrounding tissue contributes to tumor growth, invasion, angiogenesis, immune suppression, or treatment response. Their controlled conditions also make it possible to compare drug or combination-treatment effects in different microenvironmental settings. The resulting observations may identify therapeutic targets and improve prediction of how tumors respond to treatment.