Feline Oocyte Cryopreservation
Feline Oocyte Cryopreservation - Related Videos
07:16
Minimum Volume Vitrification of Immature Feline Oocytes
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Cited by 10 •
2020
This manuscript describes a protocol for the minimum volume vitrification of immature cat oocytes with laboratory-made media on commercial supports. It covers every step from oocyte isolation from ex vivo gonads to vitrification and warming.
13:36
Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos
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Cited by 9 •
2009
In this video-article we present a step-by-step demonstration on how to collect and cryopreserve hamster oocytes with high post-thaw survival rates. The same procedure can also be applied to successfully freeze and thaw mouse embryos at different stages of preimplantation development.
11:42
Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
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Cited by 8 •
2017
Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.
09:53
Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
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Cited by 11 •
2011
A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.
10:31
Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation
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Cited by 2 •
2025
This protocol describes a novel three-dimensional (3-D) culture model using a tyramine-linked hyaluronan hydrogel to encapsulate and culture preantral follicles from the mouse ovary. We also detail two approaches to ovarian follicle cryopreservation by vitrification.
FAQs
Cryoprotective solutions help prepare feline oocytes for rapid cooling by supporting formation of a glass-like state rather than damaging ice crystals. Their use is therefore central to preserving cellular structures during storage at very low temperatures. The effectiveness of this step influences whether warmed oocytes remain viable for later maturation, fertilization, or developmental study.
Rapid cooling helps limit the formation of ice crystals within or around the oocyte during vitrification. Because ice formation can damage cellular structures, achieving a glass-like state is important for retaining developmental potential. This physical principle explains why cooling conditions are a major part of preserving oocytes for subsequent laboratory procedures and developmental analysis.
Controlled warming restores the oocyte from its glass-like preserved state toward normal cellular function while supporting survival after storage. It is not simply a return to room conditions; the warming step is part of the preservation strategy. Successful warming determines whether an oocyte can proceed to in vitro maturation, fertilization, or embryo-development studies.
After warming, researchers can examine whether oocytes survive, undergo in vitro maturation, participate in fertilization, and support embryo development. These stages provide distinct information about oocyte quality and developmental competence. In developmental biology, the sequence helps connect preservation conditions with later events in maturation, fertilization, and early embryogenesis.
Following controlled warming, surviving oocytes may be placed into in vitro maturation procedures. Mature oocytes can then be subjected to fertilization, often using intracytoplasmic sperm injection, and monitored for embryo development. This workflow converts preservation into an experimental assessment of developmental potential rather than treating storage survival as the only outcome.
The method provides a way to investigate how oocyte quality and maturation relate to fertilization and early embryogenesis after preservation. Researchers can use the progression from warmed oocyte to embryo development to study developmental competence across several stages. This makes the approach relevant to questions about cellular preservation and the earliest phases of development.
Preserved feline oocytes can be incorporated into genetic resource banks, creating stored material for later fertilization and research. This is particularly relevant to conservation efforts involving endangered felids, where maintaining reproductive resources may support future developmental work. The approach therefore connects laboratory cryopreservation with longer-term management of feline genetic diversity.