Myelin Oligodendrocyte Glycoprotein
Myelin Oligodendrocyte Glycoprotein - Related Videos
03:59
Isolation and Purification of Recombinant Myelin Oligodendrocyte Glycoproteins
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2025
Source: Jain, R. W., et al. Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies. J. Vis. Exp. (2016)This video demonstrates the isolation and purification of MOGtag, a recombinant Myelin Oligodendrocyte Glycoprotein (MOG) used in Experimental Autoimmune Encephalomyelitis (EAE) studies. The protocol involves bacterial lysis, sonication, centrifugation, and nickel affinity chromatography to obtain...
10:47
Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies
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Cited by 12 •
2016
We describe a simple protocol using only basic lab equipment to generate and purify large quantities of a fusion protein that contains mouse Myelin Oligodendrocyte Glycoprotein. This protein can be used to induce experimental autoimmune encephalomyelitis driven by both T and B cells.
18:17
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues
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Cited by 106 •
2011
This article describes methods to derive enriched populations of murine oligodendrocyte precursor cells (OPCs) in primary culture, which differentiate to produce mature oligodendrocytes (OLs). In addition, this report describes techniques to produce murine myelinating co-cultures by seeding mouse OPCs onto a neurite bed of mouse dorsal root ganglion neurons (DRGNs).
04:27
An In Vitro Transduction-Induced Myelination Assay for Oligodendrocyte Precursor Cells
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2025
The video demonstrates a protocol for differentiating oligodendrocyte precursor cells (OPCs) into oligodendrocytes and inducing myelination in a co-culture with dorsal root ganglion (DRG) neurons. The OPCs are transduced with lentiviral vectors encoding a signaling protein that regulates myelination. The transduced OPCs are co-cultured with DRG neurons to assess the differentiation into oligodendrocytes and the signaling protein-induced myelination.
08:03
Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice
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Cited by 169 •
2014
Experimental autoimmune encephalomyelitis (EAE) is an established animal model of multiple sclerosis. C57BL/6 mice are immunized with myelin oligodendrocyte glycoprotein (MOG) peptide 35-55 (MOG35-55), resulting in an ascending flaccid paralysis caused by autoreactive immune cells in the central nervous system. Protocols for disease induction and monitoring will be discussed.
FAQs
Its exposed extracellular immunoglobulin-like domain can be reached by antibodies and immune cells at the outer myelin surface. This accessibility makes MOG a useful target for examining how immune recognition is directed toward myelin-associated structures. Consequently, researchers can investigate antibody-mediated injury and the cellular interactions that contribute to neuroinflammatory demyelination.
MOG can function as an antigen that focuses an immune response on oligodendrocyte-associated myelin. When antibodies recognize this accessible protein, experimental systems can be used to study demyelinating injury driven by antibody responses. This provides a defined framework for examining how loss of immune tolerance may produce inflammation and damage within the central nervous system.
MOG provides a specific self-antigen for testing how immune tolerance to central nervous system components is maintained or disrupted. Investigators can follow immune responses directed against MOG to examine why autoreactivity emerges and how it relates to neuroinflammatory disease. These studies connect antigen recognition with the mechanisms underlying autoimmune demyelination.
In experimental models, MOG serves as an antigen for inducing or examining immune responses that resemble aspects of multiple sclerosis-like demyelination. Its defined location and accessibility allow researchers to study antibody-mediated mechanisms alongside broader neuroinflammation. The models help clarify how immune targeting of myelin-associated structures can produce central nervous system injury.
Detection of MOG-specific antibodies supports the evaluation and classification of MOG antibody-associated disease. The finding helps researchers and clinicians relate an antibody response to inflammatory injury involving the brain, spinal cord, or optic nerves. It also provides a way to distinguish MOG-directed autoimmunity as a relevant pattern within broader investigations of neuroinflammatory disease.
MOG antibody-associated disease offers a defined setting for studying how autoantibodies can target an accessible protein associated with myelin-producing cells. Research on this condition links antibody specificity with injury across the brain, spinal cord, and optic nerves. It therefore strengthens understanding of immune-mediated damage and supports more precise classification of neuroinflammatory disorders.