T Cell Imaging
T Cell Imaging - Related Videos
09:24
Molecular Imaging to Target Transplanted Muscle Progenitor Cells
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Cited by 3 •
2013
A non-invasive means to evaluate the success of myoblast transplantation is described. The method takes advantage of a unified fusion reporter gene composed of genes whose expression can be imaged with different imaging modalities. Here, we make use of a fluc reporter gene sequence to target cells via bioluminescence imaging.
09:10
Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
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Cited by 8 •
2015
During postnatal cerebellum development, immature granule cells originating from the germinal zone exhibit distinct modalities of migration to reach their final destination and to establish neuronal networks. This protocol describes the preparation of cerebellar slices and the confocal macroscopic approach used to investigate the factors that regulate neuronal migration.
07:42
Live Cell Imaging during Mechanical Stretch
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Cited by 12 •
2015
A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.
15:53
Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells
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Cited by 13 •
2013
The cytotoxic activity of the phenanthridine PJ-34 in cancer cells undergoing mitosis was documented in real time by live confocal imaging. PJ-34 eradicated human breast cancer MDA-MB-231 cells harboring extra-centrosomes in mitosis. Unlike normal bi-focal mitosis, the extra-centrosomes were not clustered in the two spindle poles in the presence of PJ-34.
07:42
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
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Cited by 33 •
2008
This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.
FAQs
Imaging the immune synapse can connect molecular recognition with cell behavior. When a T cell receptor engages a peptide–MHC complex, the contact site reorganizes the cytoskeleton. Microscopy can therefore show where receptor engagement occurs alongside structural rearrangement, helping investigators relate antigen recognition to signaling organization and subsequent immune-cell interactions.
Time-lapse microscopy adds a temporal dimension to single images. It can follow whether T cells migrate toward relevant cellular targets, establish contacts, and change their behavior after antigen recognition. In infection studies, these sequential observations help distinguish movement, target engagement, and later effector activity rather than treating them as unrelated snapshots.
Fluorescent labels and reporters make otherwise dynamic cellular events observable through microscopy. Their value extends beyond locating T cells: they can associate visible changes with signaling, interactions, or structural organization. This enables experiments to compare how T cells behave when they encounter peptide–MHC complexes or infected-cell targets, while preserving a direct visual record.
The core combination is a fluorescent label or reporter plus microscopy capable of capturing the feature of interest. Live-cell imaging supports observation of ongoing behavior, while time-lapse acquisition records changes across successive moments. Together, these components allow investigators to examine migration, antigen recognition, signaling, synapse formation, and cellular interactions in a connected workflow.
By visualizing migration, signaling, antigen recognition, and interactions, T cell imaging provides direct evidence of how cellular immunity behaves rather than relying only on endpoint measurements. In vaccine research, it can help examine responses to immune stimulation; in immune dysfunction studies, altered movement, recognition, or effector behavior can become visible research outcomes.
This approach is useful when a therapy is intended to strengthen or redirect cellular immunity. Imaging can show whether treated T cells locate relevant targets, recognize antigen, organize an immune synapse, and carry out effector functions. Those observations connect therapeutic design with visible changes in T cell behavior during immune responses to infection.