Fluorescent Chimeric Mice
Fluorescent Chimeric Mice - Related Videos
11:25
Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice
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Cited by 33 •
2015
We describe a protocol whereby busulfan conditioning permits the bone marrow of a recipient mouse to be replaced with bone marrow cells from donor mice ubiquitously expressing green fluorescent protein, in the absence of irradiation. This technique is useful to study bone marrow cell accumulation in the central nervous system.
07:30
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
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Cited by 8 •
2016
This article describes how to inject viral vectors into the mouse frontal cortex to test behavioral assays that require GPCR heteromeric formation.
09:45
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
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2018
We have developed a novel method for co-expressing multiple chimeric fluorescent fusion proteins in plants to overcome the difficulties of conventional methods. It takes advantage of using a single expression plasmid that contains multiple functionally independent protein expressing cassettes to achieve protein co-expression.
21:01
Generating Chimeric Zebrafish Embryos by Transplantation
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Cited by 61 •
2009
A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.
04:38
An Ex Vivo Technique to Manufacture Chimeric Antigen Receptor T Cells
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2025
This video demonstrates a technique to manufacture chimeric antigen receptor (CAR) T cells ex vivo. Human T cells are incubated with magnetic beads coated with anti-CD3 and anti-CD28 antibodies, along with a medium supplemented with interleukin-2 (IL-2), leading to T cell activation and proliferation. Transgenic lentiviral vectors are introduced, allowing the transgenic RNA to integrate into the host genome and express the transgene. This results in the transduced T cells expressing chimeric...
FAQs
The fluorescent tag functions as an identity marker for cells introduced from a genetically distinct source. Under microscopy, researchers can distinguish these labeled cells from surrounding host tissues and follow their location, survival, and behavior in living animals. This links the cells’ origin with their changing position over time, allowing biological events to be interpreted within intact physiological environments.
Separating donor-derived cells from recipient tissues helps researchers determine whether observed biological effects arise from the introduced population, the host environment, or their interaction. In fluorescent chimeric mice, the label provides a visible way to follow the introduced cells while they participate in processes such as immune-cell trafficking, tissue regeneration, or therapeutic-cell integration.
Fluorescently labeled cells may be introduced into developing embryos or transplanted into recipient mice, creating different experimental contexts for observing cell behavior. Embryo-based introduction supports examination during development, whereas transplantation enables assessment of how introduced cells behave within an established recipient environment. The selected approach therefore depends on whether the study focuses on development, disease, or cell integration.
A typical workflow begins by obtaining cells engineered to express a fluorescent protein, such as green fluorescent protein. These cells are then introduced into developing embryos or transplanted into recipient mice. Microscopy is subsequently used to visualize the labeled population in the living organism, documenting its location, persistence, and behavior in relation to surrounding tissues.
Microscopy can reveal where labeled cells are located, whether they remain present over time, and how they behave within the recipient organism. Because observations occur in living animals, researchers can connect spatial distribution with temporal change rather than relying only on a single biological snapshot. These readouts help assess cell movement, persistence, and participation in physiological or disease-related processes.
These models are useful when researchers need to observe labeled cells during cancer growth, immune-cell trafficking, tissue regeneration, or cell transplantation. They can also support evaluation of disease mechanisms and treatment responses by showing how cells behave within complex physiological environments. For therapeutic-cell studies, visualization helps determine whether introduced cells integrate into tissues and where that integration occurs.