Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Medicine

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice
 

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

Article doi: 10.3791/52553
April 1st, 2015

Summary April 1st, 2015

We describe a protocol whereby busulfan conditioning permits the bone marrow of a recipient mouse to be replaced with bone marrow cells from donor mice ubiquitously expressing green fluorescent protein, in the absence of irradiation. This technique is useful to study bone marrow cell accumulation in the central nervous system.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter