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Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice
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Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

DOI:
10.3791/52553-v

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11:25 min

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April 01, 2015

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, , , , ,
1Department of Biomedical Physiology and Kinesiology,Simon Fraser University, 2The Biomedical Research Centre,University of British Columbia, 3Division of Neurology, Department of Medicine, Neuromuscular Disease Unit,VHHSC

Chapters

  • 00:05Title
  • 01:38Conditioning of Recipient Mice and Isolation of Donor Bone Marrow Cells
  • 06:17Bone Marrow Transplantation and Estimating the Extent of Bone Marrow Chimerism
  • 09:06Results: Quantification of Bone Marrow Chimerism in Busulfan-conditioned Mice
  • 10:49Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol whereby busulfan conditioning permits the bone marrow of a recipient mouse to be replaced with bone marrow cells from donor mice ubiquitously expressing green fluorescent protein, in the absence of irradiation. This technique is useful to study bone marrow cell accumulation in the central nervous system.

Tags

Keywords: Bone Marrow TransplantationMyeloablative ConditioningBusulfanChimerismGreen Fluorescent ProteinCentral Nervous SystemNeurodegenerationGenetic Engineering

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