Materials
Name | Company | Catalog Number | Comments |
Glass Coverslips (15mm) | Fisher Scientific | 12-545-83 | |
Gelatin 300 Bloom | Sigma-Aldrich | G-1890 | |
Tetrachloroauric Acid Trihydrate | Fisher Chemical | G54-1 | 14.5 mM (a final working solution) |
Sodium Citrate | Fisher Scientific | BP327-500 | 0.5% (a final working solution) |
Paraformaldehyde | Fisher Scientific | O4042 | 3% (a final working solution) |
100 mm Tissue Culture Dish | Sarstedt | 83.1802 | |
12-Well Plates | Fisher Scientific | 08-772-29 | |
24-Well Plates | Fisher Scientific | 07-200-84 | |
Techne Oven Hybridiser HB-1D | LabPlanet | 2040500 | The standard laboratory oven will suffice |
10 ml Serological Pipettes | Sarstedt | 86.1254.001 | |
Pipet-Aid Filler/Dispenser | Drummond | 13-681-15 | |
P200 Single-Channel Manual Pipette | Rainin | PR-200 | |
200 ml Barrier Tips | CLP | BT200 | |
ImageJ software | http://rsb.info.nih.gov/ij/ | License: Public Domain | |
Nikon Eclipse TE300 with a photometrics CoolSNAPfx monochrome 12-bit CCD camera | Nikon | Discontinued; The most comparable specification has Nikon Eclipse Ti, but a lower end Nikon 80i will be suitable as well. Other brands also provide comparable microscopes. | |
Note: The reagents and equipment listed below have been utilized by us in our various studies. Other supplies, suppliers, reagents, and equipment can be used, as long as they have similar specifications. |
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