Spectrometric Measurements
Spectrometric Measurements - Related Videos
08:40
Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure
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Cited by 5 •
2017
A reference measurement procedure for the absolute quantification of Aβ1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is described.
11:14
Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
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Cited by 19 •
2015
Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.
09:59
MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
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Cited by 14 •
2014
Mass spectrometric imaging (MSI) is a powerful tool that can be used to discover and identify various chemical species in intact tissues, preserving the compounds in their native environments, which can provide new insights into biological processes. Herein a MSI method developed for the analysis of small molecules is...
08:08
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
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Cited by 7 •
2015
In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.
13:09
Mass Spectrometric Analysis of Glycosphingolipid Antigens
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Cited by 9 •
2013
A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.
FAQs
The recorded signal may represent radiation transmitted through a sample, absorbed by it, emitted from it, or scattered from it. These signal types provide different views of the sample’s composition and physical properties. Selecting the relevant measurement mode helps researchers obtain information suited to a particular analyte, material, or biological system rather than treating every spectrum as equivalent.
Standards provide reference signals for comparing an unknown sample with materials of known composition or concentration. This comparison supports both analyte identification and quantitative estimation. In bioengineering experiments, standards help convert an observed spectral response into interpretable concentration data and make results more consistent across samples or measurement sessions.
A spectrum can provide evidence about chemical composition, concentration, and physical properties because analytes produce characteristic signals. Researchers interpret the pattern and magnitude of those signals rather than relying only on whether a signal appears. This broader information supports analysis of biological molecules, biomaterials, and changes occurring during engineered biological processes.
A typical workflow places the sample in the instrument, exposes it to selected wavelengths or a broad spectrum of light, and records the resulting radiation response. The measured spectrum is then examined and, when quantification is required, compared with standards. This sequence allows a small sample to produce data for identifying or estimating analytes.
In bioengineering, these measurements support analysis of proteins and nucleic acids by examining their characteristic spectral signals. Researchers can use the resulting data to identify or quantify these analytes, depending on the measurement and reference standards used. This makes the technique useful when biological studies require rapid analysis while conserving sample material.
Spectrometric measurements help characterize biomaterials and evaluate cell or tissue behavior by revealing changes in chemical composition or related physical properties. Their speed, sensitivity, and small-sample requirements also support monitoring enzymatic reactions and controlling research or production processes. These capabilities connect material characterization, biological evaluation, and ongoing measurement within bioengineering workflows.