Advanced Glycation End Products
Advanced Glycation End Products - Related Videos
06:05
Advanced Glycation End-Products Sensitize Human Sensory-Like Neuron Cells to Capsaicin-Induced Calcium Influx
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Cited by 1 •
2025
Increased collagen-derived advanced glycation end products (AGEs) are consistently linked to painful diseases. Here, we investigated whether glycation sensitizes sensory neurons to capsaicin excitation.
00:56
Extraction: Advanced Methods
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2024
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...
11:42
Lentivirus Production
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Cited by 43 •
2009
To make lentiviruses, DNA vectors are transfected into human 293 cells. After harvest and concentrating the supernatant, virus titer is determined by fluorescence expression with a flow cytometer.
02:22
Overview of Advanced Functional Groups
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2023
Functional groups are groups of atoms with specific chemical properties that occur within organic molecules and are sometimes denoted as “R”. Functional groups can “functionalize” a compound by enabling it to adopt different physical and chemical properties. Types of Advanced Functional Groups The table below summarizes some of the major functional groups in organic chemistry. Class of Organic Compounds Skeletal Structures of Compounds with Functional Groups in Red Names of...
10:59
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
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Cited by 32 •
2013
Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five Apolipoprotein (Apo) C-III KO rabbits using Zinc Finger Nucleases (ZFN). This work demonstrated that ZFN is a highly efficient method to produce KO rabbits.
FAQs
The sequence from Schiff base to Amadori product creates opportunities for oxidation and dehydration, which drive the formation of more stable adducts and cross-links. Because these reactions can affect proteins, lipids, and nucleic acids, the resulting AGE population is chemically diverse. This progression helps explain why AGE-related effects vary across biological molecules and tissues.
Cross-links can connect molecules that normally move or function independently, while stable adducts can alter the molecules to which they attach. In tissues, these changes may affect protein structure and tissue elasticity. At the cellular level, modified molecules can contribute to altered cellular function, making cross-link formation an important mechanism in studies of aging and disease.
AGEs can interact with RAGE, a cell-surface receptor, and activate signaling pathways associated with oxidative stress and inflammation. This receptor-mediated response differs from the direct structural effects caused by AGE adducts or cross-links. Studying both mechanisms helps biologists distinguish how chemical modification and cellular signaling together contribute to changes in tissue and cell behavior.
Measurement can indicate how extensively glycation-related products have accumulated and can support comparisons between biological conditions. Researchers may relate these measurements to changes in protein structure, tissue elasticity, or cellular function. They can also use the results to investigate disease mechanisms and to evaluate whether inhibiting AGE formation changes the associated biological outcomes.
These conditions provide biological contexts in which AGE accumulation and its consequences can be examined. Studies may connect AGE-associated molecular changes with altered tissue properties, cellular dysfunction, oxidative stress, or inflammatory signaling. Comparing these contexts helps researchers determine how the same glycation-related processes may contribute to different disease mechanisms and age-related biological changes.
Researchers can examine AGE formation under conditions where the process is reduced, then compare molecular or cellular outcomes with those observed when accumulation occurs. This approach helps test whether AGE-related changes contribute to a biological effect rather than merely accompany it. It also provides a framework for evaluating potential therapeutic strategies aimed at limiting AGE-associated damage.