Biolistic Delivery
Biolistic Delivery - Related Videos
05:33
Biolistic-Mediated Gene Transfer: A Technique to Deliver Gene of Interest in Target Cells via a Biolistic Gene Gun
0 Views •
2025
In this video, we demonstrate the protocol of biolistic gene gun-mediated gene delivery in cultured cells. This method provides a simple method of stable genetic transformation using a minimal quantity of DNA.
10:15
Generation of Transgenic C. elegans by Biolistic Transformation
0 Views •
Cited by 27 •
2010
Transgenic worms are commonly used in C. elegans research. Described is a simple, yet effective, protocol to introduce transgenes into worms using biolistic bombardment with DNA-coated gold particles. The effort involved and results of bombardment compare favorably with microinjection for the generation of transgenic animals.
07:14
A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
0 Views •
Cited by 3 •
2010
Macromolecular trafficking between plant cells can be assessed by transiently expressing a fluorescently-tagged protein of interest and analyzing its intra- and intercellular distribution by confocal microscopy.
17:42
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
0 Views •
Cited by 90 •
2008
We describe a method for preparing DNA coated gold bullets and demonstrate the use of such bullets to biolistically transfect neurons in cultured hippocampal slices.
06:40
Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun
0 Views •
Cited by 9 •
2014
Recent improvements in organotypic brain slice preparations have permitted their exploitation for biotechnological applications. Organotypic slices maintain local structural characteristics of in vivo biology, including functional synaptic connections. Here we present a regioselective biolistic delivery method to label and genetically manipulate these slices.
FAQs
Gold or tungsten particles act as physical carriers for DNA and are accelerated toward the target tissue. Their movement allows the genetic material to pass through cellular barriers that may limit other delivery approaches. This physical entry mechanism is especially useful when the specimen is difficult to transfect by chemical or viral methods.
The outcome depends on how the introduced DNA behaves after it enters the cells. Transient expression provides temporary activity from the delivered genetic material, whereas stable expression reflects longer-term maintenance of the introduced genetic information. These alternatives allow experiments to examine short-term gene function or support the development of engineered organisms.
Its physical delivery strategy does not depend on the same chemical uptake processes or viral systems used by other approaches. That makes it valuable for specimens that are difficult to transfect through those routes. In biology, this flexibility extends genetic engineering to target tissues and cell types that might otherwise be challenging to manipulate.
A typical workflow begins by coating microscopic gold or tungsten particles with DNA. The coated particles are then accelerated into the selected tissue or specimen, allowing the DNA to enter cells. Researchers subsequently assess whether the material supports transient or stable gene expression, depending on the purpose of the experiment.
Plant cells and embryos are important targets, along with other specimens that are difficult to transfect. The method can also support genetic manipulation involving chloroplasts and mitochondria, extending its use beyond general cellular transformation. This range makes it relevant to studies that require delivery into specialized tissues or cellular compartments.
Biolistic delivery can enable functional studies by introducing genetic material whose activity researchers can examine in target cells. It also contributes to the production of engineered organisms and the development of gene-based tools. Applications involving chloroplasts and mitochondria provide additional opportunities to investigate or modify genetic processes associated with these cellular structures.