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A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Article DOI: 10.3791/2208 • 07:14 min • August 27th, 2010
August 27th, 2010 •
1, 1, 2, 2
1Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, 2Bio-Medical Engineering Department, NED University of Engineering and Technology

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Summary
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Macromolecular trafficking between plant cells can be assessed by transiently expressing a fluorescently-tagged protein of interest and analyzing its intra- and intercellular distribution by confocal microscopy.

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Cell-to-cell Macromolecular Transport Assay Biolistic Bombardment Fluorescently Tagged Protein Encoding DNA Construct Plant Tissue Confocal Microscopy Symplastic Protein Transport Arabidopsis Thaliana Nicotiana Benthamiana N. Tabacum (tobacco)
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