Long Term Culture
Long Term Culture - Related Videos
03:41
In Vitro Calcium Imaging in Short-Term and Long-Term Rat Hippocampal Cultures
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2025
Source: Calvo-Rodríguez, et al. Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons. J. Vis. Exp. (2015). This video demonstrates the use of fluorescence imaging to measure cytosolic calcium levels in short-term and long-term rat hippocampal cultures. By comparing young and aged neurons, it highlights how increased L-type voltage-gated calcium channel density and prolonged activation in aged neurons result in calcium overloading, a hallmark of neuronal aging.
05:23
A Modified Roller Tube Method for the Long-Term Culture of Rodent Brain Slices
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2025
This video demonstrates a modified roller tube method for the long-term culturing of brain slices from a rodent model. First, the brain slices are attached to coverslips. Then, flat-sided roller tubes with a hole on the flat side are taken, and the coverslips are positioned on the hole using adhesive discs. Finally, a nutrient medium and a carbon dioxide and air mixture are added to the tube, and the tubes are incubated with a rolling motion.
10:10
Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
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Cited by 11 •
2011
We have developed a collagen-based in vitro assay which promotes proliferation and invasion from samples of all breast cancer subtypes. Optical Projection Tomography, a three dimensional microscopy technique was utilised to visualise and quantify tumour expansion. This assay may be used to quantify drug response of individual tumour samples.
01:05
Long-term Depression
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2019
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory. Calcium Ion Concentration Mechanism If over time, all...
11:19
A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
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Cited by 45 •
2016
Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.
FAQs
Stable nutrients, temperature, and gas levels help preserve the conditions cells need to remain viable and biologically active. As culture time increases, resource availability and environmental balance can affect proliferation, differentiation, and cellular stress. Controlling these variables allows researchers to distinguish genuine biological responses from changes caused by unsuitable laboratory conditions.
Subculturing refreshes resources and limits stress associated with excessive cell density. A defined density provides a consistent point for transferring cells into fresh culture conditions, helping maintain comparable growth behavior between experimental periods. This timing is important because inconsistent culture density can influence proliferation and other cellular characteristics, reducing the reliability of comparisons.
Extended culture can reveal contamination, genetic instability, or alterations in cell characteristics that may not be apparent during shorter experiments. These changes can affect how cells grow, differentiate, or respond to treatments. Regular monitoring and quality control are therefore essential for recognizing when observations reflect the intended biology rather than deterioration or adaptation within the culture.
Maintenance requires sterile handling, suitable nutrients, controlled temperature and gas levels, and regular observation of the culture. Researchers monitor cellular condition and density, then subculture when the defined density is reached to restore resources and reduce stress. Consistent control of these steps supports continued growth while helping identify contamination or other changes that could compromise results.
This approach is useful when researchers need to examine biological behavior across extended periods rather than at a single time point. It can support studies of cell proliferation, differentiation, disease progression, and responses to treatments. Observing these processes over time may reveal changes in cellular behavior that short-duration experiments would not capture.
Quality control helps determine whether experimental findings arise from the intended biological process or from problems developing during culture. Checking for contamination, genetic instability, altered cell characteristics, and unsuitable conditions strengthens result interpretation. Careful monitoring also improves reproducibility, allowing observations from separate culture periods or experiments to be compared with greater confidence.