Spirochetes Pleomorphic Bacteria
Spirochetes Pleomorphic Bacteria - Related Videos
02:28
Bacteriophage-Mediated Gene Delivery in Spirochete Bacteria
0 Views •
2026
Source: Eggers, C. H. Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi. J. Vis. Exp. (2022).This video demonstrates the transduction of genetically modified Borrelia spirochetes using polyethylene glycol–precipitated, non-infectious bacteriophages carrying a kanamycin-resistance plasmid. After incubation with the bacteria, the culture is plated on antibiotic-supplemented medium. Only cells that receive the plasmid survive and form colonies. These colonies...
03:25
Cultivation of Pathogenic Spirochetes to Investigate Skin Infection
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2026
Source: Berthold, A., et al. Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia. J. Vis. Exp.(2022)This video demonstrates the cultivation of pathogenic spirochetes from infected skin tissue to model dermal infection. It highlights their localization in the dermis, interaction with the extracellular matrix, and survival during selective culture conditions.
09:45
Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete
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Cited by 4 •
2025
This protocol describes in vitro cultivation of the syphilis pathogen Treponema pallidum subsp. pallidum in co-culture with mammalian cells. The method is scalable; it can be used to produce large quantities of T. pallidum and to generate clonal cultures.
09:01
Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi
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Cited by 1 •
2022
The ability of bacteriophage to move DNA between bacterial cells makes them effective tools for the genetic manipulation of their bacterial hosts. Presented here is a methodology for inducing, recovering, and using φBB-1, a bacteriophage of Borrelia burgdorferi, to transduce heterologous DNA between different strains of the Lyme disease spirochete.
13:20
Detection of Bacteria Using Fluorogenic DNAzymes
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Cited by 18 •
2012
We have recently reported a novel approach for generating fluorogenic DNAzyme probes that can be applied to set up a simple, "mix-and-read" fluorescent assay for bacterial detection. These special DNA probes catalyze the cleavage of a chromophore-modified DNA-RNA chimeric substrate in the presence of crude extracellular mixture (CEM) produced by a specific bacterium, thereby translating bacterial detection into fluorescence signal generation. In this report we will describe key experimental...
FAQs
Periplasmic flagella, or endoflagella, occupy the space between the cell membrane and outer sheath. When these structures rotate, they bend and twist the cell body, producing a corkscrew-like motion. This arrangement allows spirochetes to move through liquid or viscous environments, linking the internal location of the motility apparatus directly to the cell’s characteristic movement.
Cell shape provides clues about how bacterial structure supports survival and movement. Spirochetes maintain a slender, spiral form associated with corkscrew motility, whereas pleomorphic bacteria may vary in shape and size under different conditions. Comparing these traits helps biologists distinguish structural strategies and interpret how morphology relates to bacterial behavior.
Pleomorphic bacteria may display different shapes and sizes under different conditions, so a single microscopic appearance may not represent a fixed form. Biologists must consider this variability when classifying cells and interpreting observations. Recognizing condition-dependent morphology prevents shape differences from being treated automatically as evidence of unrelated bacterial types.
Researchers examine cell form and movement through microscopic observations, then relate those findings to bacterial classification and function. For spirochetes, spiral morphology and corkscrew movement provide structural clues; for pleomorphic bacteria, variation in shape and size becomes an important observation. This approach connects visible cellular traits with questions about adaptation and survival.
Studying spirochetes can help investigate how bacterial structure contributes to movement through viscous environments, host colonization, and disease mechanisms. Their specialized motility arrangement offers a way to examine relationships between cell architecture and behavior. These questions are relevant to biology because they connect microscopic structure with interactions between bacteria and their surroundings.
Variation in bacterial shape and size can provide evidence that cellular form changes in response to different conditions. In biology, this makes pleomorphism useful for examining how bacteria adapt to their environments and how morphology affects survival. It also supports broader comparisons between structural flexibility and more specialized forms, such as the spiral organization of spirochetes.