Biochip Analysis
Biochip Analysis - Related Videos
14:53
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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Cited by 4 •
2014
We present a microfluidic-based electrochemical biochip for DNA hybridization detection. Following ssDNA probe functionalization, the specificity, sensitivity, and detection limit are studied with complementary and non-complementary ssDNA targets. Results illustrate the influence of the DNA hybridization events on the electrochemical system, with a detection limit of 3.8 nM.
11:19
Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
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Cited by 12 •
2016
This manuscript describes how to prepare fluidic biochips with Rainbow trout gill epithelial cells for use in a field portable electric cell-substrate impedance sensor. The protocol for running a rapid drinking water toxicity test with the sensor is also described.
08:03
Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
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Cited by 3 •
2013
All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.
07:10
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
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Cited by 5 •
2019
This paper presents a protocol for detecting serum-specific immunoglobulin E with a microfluidic cartridge-based chemiluminescence system and the evaluation of its usefulness in allergy diagnoses.
15:04
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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Cited by 46 •
2009
This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
FAQs
Capture molecules establish the assay’s molecular selectivity. Antibodies, antigens, nucleic acid probes, and receptors are immobilized on the chip, where each can bind a corresponding target in the sample. The resulting binding event is converted into an optical, electrical, or chemical signal, allowing the platform to distinguish selected immune or infection-related molecules from other sample components.
Multiplexing allows multiple immune or infection-related targets to be examined in parallel on one small platform. This can reveal patterns rather than a single isolated measurement, such as a broader immune response or a set of biomarkers associated with infection. The approach also supports higher-throughput analysis while conserving sample, which is useful when material is limited.
The capture chemistry determines which interaction the assay reports. Antibodies or antigens support analysis of antigen-antibody relationships, while receptors can examine receptor-target binding. Nucleic acid probes provide a different recognition route for biological targets. Choosing among these components therefore connects the biochip readout to questions about immune responses, pathogen detection, biomarkers, or host-pathogen interactions.
A basic workflow starts by selecting immobilized capture molecules that match the biological question. The sample is then applied so relevant targets can bind, after which the chip’s optical, electrical, or chemical response is measured. Multiple capture regions can be read together, producing parallel results for comparing targets, immune signals, or infection-associated markers within the same analysis.
It is especially useful when investigators need to examine many targets but have only a small sample volume. In immunology and infection research, that combination supports broad immune-response profiling, biomarker assessment, and testing for infectious agents without requiring a separate platform for every target. The parallel format can also accelerate comparisons among samples or molecular interactions.
For infectious disease work, biochip analysis can detect infectious agents and measure biomarkers or immune responses associated with them. Comparative measurements can contribute to pathogen surveillance, while rapid parallel readouts may support diagnostic investigations. The same platform can also compare antigen-antibody interactions, linking detection-oriented results with information about how host defenses recognize infection.
In host-pathogen research, the relevant output is not limited to whether a target is present. Researchers can profile immune responses, examine antigen-antibody interactions, and investigate biomarkers in relation to infectious agents. Reading these measurements together helps connect molecular recognition with broader questions about host defense and pathogen-associated biology, while retaining the platform’s parallel analytical format.