Dendritic Cell Analysis
Dendritic Cell Analysis - Related Videos
07:59
The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
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Cited by 15 •
2012
We present a protocol that permits to view and to quantitatively asses the morphology of the dendritic tree of individual Purkinje cells grown in organotypic cerebellar slice cultures. This protocol is intended to promote studies on the mechanisms of Purkinje cell dendritic development.
07:52
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
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Cited by 1 •
2012
We describe a strategy to monitor maturation and migration of pulmonary dendritic cells in response to ovalbumin in the setting of ovalbumin induced allergic airway inflammation. This strategy can be modified to assess migration of pulmonary dendritic cells in settings of infection.
03:35
Flow Cytometry-Based Analysis of Dendritic Cell Activation Using Immune Complexes
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2025
This video demonstrates a protocol for assessing the activation of dendritic cells using immunoglobulin G (IgG)-coated tumor cells. Dendritic cells internalize the IgG-coated tumor cells, process the tumor antigens, and present them on the surface through MHC-II molecules, along with the co-expression of the costimulatory molecule CD86. These dendritic cells then undergo MHC-II and CD86-targeted immunostaining and flow cytometry analysis to identify their activation state.
11:48
Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
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Cited by 69 •
2011
Numerous recent studies have identified mutations in synaptic proteins associated with brain pathologies. Primary cultured cortical neurons offer great flexibility in examining the effects of these disease-associated proteins on dendritic spine morphology and motility.
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
FAQs
Surface-marker profiling helps researchers evaluate dendritic cell identity and phenotype rather than relying on a single functional measurement. Comparing marker patterns across isolated tissue or cultured cells can indicate differences in cellular state and support standardized comparisons between experimental conditions. This approach is especially useful when infection or other stimuli may alter the characteristics of the analyzed population.
Antigen uptake and maturation describe connected stages of dendritic cell activity. Uptake shows whether cells acquire material for immune processing, while maturation indicates a functional change associated with their ability to engage adaptive immunity. Measuring both provides a more informative picture than either readout alone and helps clarify how experimental conditions influence the transition toward antigen presentation and T-cell priming.
Analysis can show how pathogens influence dendritic cell activation, cytokine production, antigen presentation, and interactions with T cells. These readouts help distinguish effects that support host defense from those associated with immune evasion or excessive inflammation. Examining several functions together therefore connects cellular observations with broader questions about infection outcome and the regulation of innate and adaptive immunity.
A typical workflow begins by obtaining dendritic cells from tissues or cultures, followed by assessment of identity and phenotype. Researchers then examine selected functions, such as antigen uptake, maturation, cytokine production, or antigen presentation, using complementary assays. Combining these stages allows cellular characteristics and functional behavior to be interpreted together rather than treated as isolated measurements.
Flow cytometry supports systematic assessment of cellular markers and selected phenotypic features, whereas microscopy provides visual information about cells and their interactions. Functional assays test activities such as uptake, cytokine production, or antigen presentation. Using these methods together strengthens interpretation because phenotype, appearance, and immune function can be compared within the same experimental framework.
The approach is useful when investigators need to compare immune responses across disease models, characterize pathogen effects, or evaluate vaccine responses and immunotherapies. It can connect dendritic cell behavior with T-cell priming, host defense, inflammation, and immune evasion. Standardized profiling also makes results easier to compare across experiments, supporting mechanistic studies and translational research questions.