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Immunology and Infection

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JoVE Journal Immunology and Infection
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
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Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

Article DOI: 10.3791/4014 • 07:52 min • July 23rd, 2012
July 23rd, 2012 •
1, 2
1Stem Cell and Cancer Research Institute, McMaster University, Hamilton, 2Physiology and Experimental Medicine Research Program, Hospital for Sick Children, University of Toronto

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Summary
Automatic Translation

We describe a strategy to monitor maturation and migration of pulmonary dendritic cells in response to ovalbumin in the setting of ovalbumin induced allergic airway inflammation. This strategy can be modified to assess migration of pulmonary dendritic cells in settings of infection.

Tags

Pulmonary Dendritic Cells Maturation Migration Allergic Airway Inflammation Adaptive Immune Response Antigen-specific T Cells Peripheral Tissues Antigen Stimulation Draining Lymph Nodes T Cell Response Autoimmune Response Allergic Immune Response Immune Tolerance Lung Environment Environmental Antigens Allergy Asthma Airway DC Maturation Airway DC Migration Sensitization Ovalbumin-induced Allergic Asthma
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