Genexpert Dx System
Genexpert Dx System - Related Videos
08:10
Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
0 Views •
Cited by 48 •
2012
The Xpert MTB/RIF test integrates sample decontamination, hands-free operation, on-board sample processing, and ultra-sensitive hemi-nested PCR for the simultaneous detection of Mycobacterium tuberculosis and rifampicin resistance, either in expectorated sputum or concentrated sputum sediments, in approximately two hours. Testing is standardized and requires only moderate laboratory infrastructure and training.
08:24
Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling
0 Views •
Cited by 5 •
2008
The Ugi reaction has proved to be a convenient way to quickly create diverse libraries of compounds. It involves the reaction of an amine, an aldehyde, a carboxylic acid and an isonitrile typically in methanol at room temperature. In this video, we utilize a 48-slot Mettler-Toledo MiniBlock equipped with filtration tubes and a Mettler-Toledo MiniMapper automated liquid handler was used to deliver the reagents and solvent. The parameters of interest were the concentration, the solvent...
09:18
Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
0 Views •
Cited by 6 •
2015
Transporters in cell membranes allow differential segregation of ions across cell membranes or cell layers and play crucial roles during tissue physiology, repair and pathology. We describe the ion-selective self-referencing microelectrode that allows the measurement of specific ion fluxes at single cells and tissues in vivo.
16:40
A New Single Chamber Implantable Defibrillator with Atrial Sensing: A Practical Demonstration of Sensing and Ease of Implantation
0 Views •
Cited by 5 •
2012
Dual-chamber implantable cardioverter-defibrillators (ICDs) may improve detection of atrial fibrillation as well as differentiation of tachycardias. However, this advantage is undermined by complications associated with the second electrode, which is required in conventional dual chamber devices. Therefore, BIOTRONIK has developed a new electrode called the LinoxSMART S DX that, when used in conjunction with the Lumax DX ICD, offers dual-chamber detection without the risks associated with the...
09:59
Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
0 Views •
Cited by 3 •
2013
Autophagy is a ubiquitous process that enables cells to degrade and recycle proteins and organelles. We apply advanced fluorescence microscopy to visualize and quantify the small, but essential, physical changes associated with the induction of autophagy, including the formation and distribution of autophagosomes and lysosomes, and their fusion into autolysosomes.
FAQs
The workflow links several analytical stages inside a disposable cartridge. The system processes the specimen, purifies nucleic acids, amplifies selected targets through real-time polymerase chain reaction, and follows fluorescence during amplification. The resulting signal pattern supports qualitative detection or quantitative measurement, allowing the instrument to report molecular findings without requiring separate preparation, amplification, and detection workflows.
Real-time PCR provides the amplification step needed to detect clinically relevant genetic material. As target nucleic acids are amplified, fluorescent signals are monitored rather than evaluated only after the reaction is complete. This integrated measurement enables the system to distinguish whether a target is detected and, when the assay supports it, provide a quantitative result.
The cartridge contains specimen processing, nucleic-acid purification, amplification, and signal detection within a standardized workflow. Enclosure limits the need to transfer material between separate stages, while standardization reduces variation caused by hands-on manipulation. Together, these features promote reproducible testing and help limit contamination risk, which is especially relevant when handling infectious specimens or amplified nucleic acids.
A specimen is processed through a disposable cartridge, where target nucleic acids are purified and then amplified by real-time PCR. The instrument monitors fluorescence during amplification and converts the measured signal into a qualitative or quantitative result. Because these stages are integrated, the operator has limited hands-on manipulation compared with workflows requiring separate preparation, amplification, and detection steps.
The platform is useful when investigators or clinicians need molecular evidence of an infectious agent or a clinically relevant antimicrobial-resistance marker. Its integrated workflow supports rapid testing while reducing manual processing. Results can then be considered alongside disease status and treatment decisions, making the system relevant to both infection diagnostics and research that connects pathogen detection with clinical interpretation.
In this subject area, molecular findings can be connected with the broader status of an infectious disease rather than viewed as isolated laboratory signals. Detection of a pathogen or resistance-associated genetic target may help investigators examine disease-related patterns and treatment relevance. The platform contributes standardized, reproducible measurements that support this connection while limiting contamination-prone handling steps.