Immune Cell Quantification
Immune Cell Quantification - Related Videos
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
02:55
Quantification of Immune Mediator Levels in Human Mucosal Lining Fluid
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2025
This video demonstrates the quantification of immune mediator levels in neonatal human nasal mucosal lining fluid extract. An electrochemiluminescence-based multiplex assay system measures these mediators by evaluating the intensity of emitted light when they are excited.
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
11:00
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
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Cited by 14 •
2020
Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays.
05:07
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
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Cited by 30 •
2016
We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.
FAQs
Fluorescent antibodies bind selected cell-surface or intracellular markers, allowing flow cytometry to identify immune cell populations within a biological sample. Using different markers provides information about which cells are present and can support assessment of immune activation. The resulting measurements help researchers separate changes in cell composition from broader changes in total sample cellularity.
Proportions describe how much of a sample belongs to a particular immune cell population, but they do not by themselves establish the number of cells present. Counting beads or a calibrated sample volume provides a reference for converting detected events into absolute counts. This distinction helps identify whether disease or treatment is associated with cell expansion, depletion, or redistribution.
The methods differ in how they generate measurements and which samples or cell types they best address. Flow cytometry uses fluorescent markers, microscopy provides an imaging-based approach, automated hematology analysis supports cellular measurement, and nucleic acid-based assays measure nucleic-acid signals. Researchers select among them according to the cell type, biological sample, and information required.
A proportion and an absolute count describe different features of an immune response. A population may represent a larger or smaller share of the sample, while the total number of those cells may change through recruitment, depletion, or expansion. Considering both measurements therefore gives a more complete view of altered immune status during infection or other disease-related processes.
A typical measurement uses a biological sample, fluorescent antibodies directed against cell-surface or intracellular markers, and a counting reference such as beads or a calibrated sample volume. The markers identify the relevant cell populations, while the reference supports absolute enumeration when needed. This combination produces either proportional measurements, absolute counts, or both, depending on the study design.
Microscopy and automated hematology analysis are related alternatives when the sample or cell type makes another measurement strategy appropriate. Their selection depends on the biological material and the information sought, while nucleic acid-based assays provide another option for samples where cellular measurement is not the preferred approach. These methods broaden how immune populations can be investigated beyond one platform.
Measurements of immune cell populations can show leukocyte recruitment, depletion, expansion, and immune activation during infection or disease. Researchers use these patterns to characterize host responses and follow disease progression. The same measurements can also help evaluate vaccines or antimicrobial treatments by revealing how immune status changes during an intervention.