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JoVE Journal
Immunology and Infection

This content is Open Access.

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
 

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Article doi: 10.3791/60740
March 25th, 2020

Summary March 25th, 2020

Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays.

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