Immune Cell Tracking
Immune Cell Tracking - Related Videos
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
22:49
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
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Cited by 45 •
2012
Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
05:07
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
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Cited by 30 •
2016
We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.
07:09
Isolation of Adipose Tissue Immune Cells
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Cited by 75 •
2013
Adipose tissue (AT) is a site of intense immune cell activation and interaction. Almost all cells of the immune system are present in AT and their ratios are altered by obesity. Proper isolation, quantification, and characterization of AT immune cell populations are critical for understanding their role in immunometabolic disease.
FAQs
Label choice determines what aspect of movement can be followed. Fluorescent dyes provide detectable signals for microscopy or flow cytometry, whereas reporter genes and molecular probes offer alternative ways to mark cells for observation. The resulting signal can be followed alongside cell location and persistence, helping investigators connect a cell’s movement with its behavior during an immune response.
Detection method shapes the scale and type of information obtained. Microscopy can show immune cells in relation to infected tissues or neighboring cells, flow cytometry can identify labeled populations together with antibody-based markers, and whole-body imaging can follow signals across the organism. The choice depends on whether the study emphasizes cellular interactions, population identity, or distribution.
Persistence is interpreted together with migration and state changes rather than as an isolated measurement. Tracking can show whether cells move from blood into infected tissue, remain present, interact with pathogens or other cells, and change in activation or survival. These linked observations help distinguish simple arrival from a sustained or altered immune response.
A typical study begins by choosing a cell label and an appropriate detection platform, then using the resulting signal to locate or identify cells during the relevant immune response. Researchers may add antibody-based markers when they need to distinguish particular populations. Comparing detected cells across locations or time points can reveal migration, persistence, interactions, activation, or survival changes.
Location alone does not indicate whether a cell is contributing to an effective response. Pairing spatial or distribution information with antibody-based identification and signals associated with activation or survival allows investigators to relate where a population is found to how it is behaving. In infection studies, that connection supports analysis of host defense and disease progression.
It can examine host defense, disease progression, vaccine responses, and the effects of immunotherapies. The same framework can also reveal how immune cells interact with pathogens or with other cells. These applications make migration and persistence measurable in contexts where the central question is how immune-cell behavior changes during an immune response.