Natural Product Screening
Natural Product Screening - Related Videos
08:10
A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix
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Cited by 42 •
2013
The article describes a methodology for the production of an acellular matrix from rat intestine. The derivation of intestinal scaffolds is important for future applications in tissue engineering, stem cell biology and drug testing.
10:24
A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources
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2026
This protocol introduces a fluorometric screening methodology optimized for identifying natural product/small molecule inhibitors of protein synthesis. Its practicality makes it suitable for both undergraduate instruction in drug discovery techniques and implementation in medicinal chemistry campaigns.
07:28
Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
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Cited by 4 •
2020
The elucidation of the mode-of-action of a novel antibiotic is a challenging task in the drug discovery process. The goal of the method described here is the application of isothermal microcalorimetry using calScreener in antibacterial profiling to provide additional insight into drug-microbe interactions.
12:15
Generation of Monoclonal Antibodies Against Natural Products
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Cited by 2 •
2019
This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided.
09:04
A Direct, Early Stage Guanidinylation Protocol for the Synthesis of Complex Aminoguanidine-containing Natural Products
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Cited by 3 •
2016
Here, we present a protocol for direct, early stage guanidinylation that enables rapid total synthesis of aminoguanidine-containing small organic molecules. An advanced synthetic intermediate used in the synthesis of a blood coagulation factor XIa inhibitor was prepared using this protocol.
FAQs
Concentration comparisons help distinguish consistent biological responses from isolated observations. Researchers expose a defined assay system to multiple concentrations and evaluate the results alongside appropriate controls. This design shows whether antimicrobial or immunomodulatory activity changes across the tested range, providing stronger evidence that a natural source or compound produces a measurable effect rather than an incidental result.
Activity-guided purification links an observed response to the constituent responsible for it. Researchers separate an active extract into fractions, test those fractions, and continue purification while tracking the biological effect. This step narrows a complex mixture to active constituents and creates material suitable for target or pathway assays that can clarify how the activity occurs.
A natural product may act directly on infection by inhibiting pathogen growth or disrupting virulence. Another may primarily influence the host by regulating inflammatory signaling or enhancing host defense. These outcomes represent distinct biological effects, so immunology and infection studies should examine both pathogen-related responses and immune-related responses when characterizing activity.
The workflow begins with plant, microbial, fungal, or other natural material and preparation of an extract or purified compound. Researchers then expose a defined assay system to test concentrations with appropriate controls. Active results prompt fractionation and activity-guided purification, followed by target or pathway assays to validate the constituent and investigate its mechanism.
Defined assays can reveal whether a tested material inhibits pathogen growth, affects virulence, changes inflammatory signaling, or enhances host defense. These outcomes help separate direct anti-infective effects from immune-modulating effects. Comparing responses across concentrations and against controls also supports interpretation of whether the activity is reproducible and biologically meaningful within the assay system.
This approach is useful when researchers seek new substances with anti-infective or immune-modulating potential. Screening can identify candidates that act against pathogens, interfere with virulence, regulate inflammation, or strengthen host defense. Subsequent purification and mechanism-focused assays help prioritize active constituents for further investigation and support the broader search for therapeutic leads.