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JoVE Journal
Immunology and Infection
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Generation of Monoclonal Antibodies Against Natural Products
 

Generation of Monoclonal Antibodies Against Natural Products

Article doi: 10.3791/57116
April 6th, 2019

Summary April 6th, 2019

This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided.

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