A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources

A fluorescence-based protocol for preliminary screening of protein synthesis inhibitors from natural sources. Protein synthesis involves highly regulated processes, transcription and translation. Transcription is primarily regulated by transcription factors, chromatin modifications, and regulatory RNAs.

While translation is regulated by initiation factors, signaling pathways and RNA stability mechanisms. These regulatory systems allow cells to maintain cellular functions and respond to stress or damage effectively. Cancer cells dysregulate protein synthesis by hijacking translational machinery to support their high energy demands and uncontrolled growth.