Same-day Results
Same-day Results - Related Videos
11:16
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
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Cited by 59 •
2009
This video shows a procedure for isolating intact protoplasts from tissues of 14-day-old seedlings of Arabidopsis. Given that the isolated protoplasts remain intact for at least 96h and are isolated from seedlings instead of one-month-old mature plants, this procedure expedites assays requiring intact protoplasts.
04:06
Identification of Pre-weanling 7-Day-Old Mice with Pinna Edge Biopsies
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2025
The protocol presents a novel stepwise approach to producing a pinna-edge biopsy (PEB) in post-natal day (PND) 7 mice for the purpose of identification and simultaneous genotyping.
08:49
Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
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Cited by 5 •
2020
We outline a 9-day protocol for the transduction and expansion of rhesus macaque peripheral blood mononuclear cells which yields cells with excellent co-expression of the genes of interest in sufficient number for infusion studies of cell efficacy.
10:16
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
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Cited by 10 •
2016
This protocol describes an accurate, inexpensive, rapid and non-toxic method to determine the sex of Day 30 porcine embryo using PCR method after grinding an embryo into powder without phenol chloroform extraction and DNA column purification.
06:10
Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates
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Cited by 3 •
2022
For the observation of murine neonatal bile duct disorders, an intact bile duct and efficient preparation are required. Therefore, a new approach for isolating the entire extrahepatic bile duct system in murine neonates was successfully developed while maintaining the integrity of the bile duct.
FAQs
Rapid assays shorten reporting by combining streamlined specimen preparation with accelerated detection. Depending on the assay, the measured target may be pathogen nucleic acid, a pathogen antigen, or a host-derived signal such as an antibody or inflammatory marker. This target choice determines whether the result primarily informs evidence of infection or the associated immune response.
Interpretation depends on more than how quickly a finding is reported. Assay performance affects confidence in the measurement, while specimen quality can influence whether the target is detected reliably. Timing also matters because pathogen signals and host responses may change during infection. Consequently, a same-day result supports decision-making but does not eliminate the need for contextual interpretation or, when appropriate, confirmatory testing.
Pathogen and host measurements answer different scientific questions. Detecting nucleic acids or antigens provides information about the infectious agent, whereas antibodies and inflammatory markers describe aspects of the host response. Using these categories appropriately helps investigators separate evidence about infection from evidence about immune activation, which is important when evaluating disease progression or changing responses in immunology and infection studies.
Changing immune responses make the timing of sampling especially important. A host antibody or inflammatory-marker finding reflects the state of the response at collection, not necessarily the entire course of infection. Comparing findings collected at different times can therefore help researchers evaluate response changes, provided they account for assay performance and specimen quality when interpreting differences between results.
The workflow depends on collecting a suitable clinical or research specimen, applying streamlined preparation, performing an accelerated detection assay, and reporting the finding on the collection day. The specific target may be nucleic acid, antigen, antibody, or inflammatory marker. Because specimen quality and assay performance affect interpretation, rapid processing should be paired with appropriate review.
They can help clinicians and study teams decide whether to pursue confirmatory testing, begin or adjust treatment, implement isolation, or monitor a patient more closely. The value lies in reducing delays between sample collection and an actionable finding. These results do not replace interpretation of assay performance, specimen quality, or testing time, but they can make next steps more timely.
In outbreak response, rapid reporting can help teams act on emerging infection information without waiting for extended laboratory delays. In clinical trials, it can streamline workflows that depend on timely assessment of infection or immune responses. The same-day format is therefore useful both for operational decisions and for tracking study-related findings, while interpretation still requires attention to test and specimen limitations.