Rodent Electrophysiology
Rodent Electrophysiology - Related Videos
08:22
Simultaneous fMRI and Electrophysiology in the Rodent Brain
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Cited by 45 •
2010
We have developed a method for simultaneous functional magnetic resonance imaging and electrophysiological recording in the rodent brain, providing a platform for the investigation of the relationship between neural activity and the blood oxygenation level dependent (BOLD) MRI signal.
02:53
Electrophysiological Recordings of Brainstem-Spinal Cord Preparations from a Newborn Rodent
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2025
This video demonstrates electrophysiological recordings of isolated brainstem-spinal cord preparations from newborn rodents. The preparation is placed in a recording chamber, and the inspiratory bursts from the brainstem are recorded under oxygenated and oxygen-deprived conditions using the fourth ventral nerve root in the spinal cord.
10:46
Non-fluoroscopic Catheter Tracking for Fluoroscopy Reduction in Interventional Electrophysiology
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Cited by 4 •
2015
Radiation exposure is an underestimated risk in complex ablation procedures. Here, we describe a protocol to significantly decrease fluoroscopy time and dosage for both the patient and the lab staff by using a novel non-fluoroscopic catheter visualization system.
09:58
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
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Cited by 31 •
2018
Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
05:28
Electrophysiology on Isolated Brainstem-spinal Cord Preparations from Newborn Rodents Allows Neural Respiratory Network Output Recording
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Cited by 6 •
2015
The central respiratory drive is located in the brainstem. Spontaneous respiratory motor output from an isolated brainstem-spinal cord is recorded by placing an electrode on the fourth ventral root. This experimental approach is valuable for pharmacological investigations or the assessment of respiratory challenges and genetic manipulations on rhythmic motor behavior.
FAQs
Action-potential recordings focus on brief voltage changes produced by individual neurons, whereas collective signals represent activity shared across groups of cells or larger tissue regions. This distinction determines the level of analysis: researchers can examine cellular firing patterns or assess coordinated circuit activity. Selecting the signal type helps match the recording to questions about communication, integration, or network function.
Living-animal recordings allow electrical activity to be related to stimuli, behavior, and experimental conditions within an intact organism. Isolated preparations provide access to brain tissue in a more controlled setting, which can support focused examination of cellular or synaptic function. Comparing these approaches helps researchers distinguish circuit activity linked to behavior from mechanisms that can be studied directly in tissue.
Researchers interpret changes in electrical activity alongside sensory stimuli, behavioral responses, or other experimental conditions. Patterns in firing or collective signals can then be compared with what the animal experiences or does. This approach connects measurable neural events with circuit-level processing, allowing studies to examine sensory function, learning-related activity, and changes in information handling.
Electrophysiological measurements become more informative when combined with behavioral testing, pharmacology, or targeted neural manipulation. A behavioral task links activity to function, pharmacology examines responses under altered chemical conditions, and targeted manipulation tests whether a selected neural component contributes to the observed signal. Together, these approaches help move from correlation toward circuit-level interpretation.
A typical experiment requires a rat or mouse, electrodes, a selected recording setting, and an experimental condition that can be related to the measured activity. The recording may occur in a living animal or an isolated preparation, and investigators may pair it with stimuli, behavior, pharmacology, or neural manipulation. These elements define what the resulting electrical data can address.
It is useful when researchers need to connect electrical activity with changes in communication between neurons or with behaviorally relevant processing. Recordings can characterize synaptic function in brain tissue and track activity during learning-related experiments. When paired with behavioral testing or targeted manipulation, the method helps relate cellular mechanisms to broader circuit and systems-level changes.
Recordings can identify disease-related changes in electrical activity, synaptic function, or circuit behavior in rats and mice. Because the measurements can be linked to cellular signals, neural circuits, and observable behavior, they provide several levels for comparing altered and typical function. Combined with targeted manipulation or pharmacology, the approach also supports evaluation of mechanisms relevant to treatment development.