Thy1-yfp Mice
Thy1-yfp Mice - Related Videos
10:03
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
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Cited by 11 •
2012
Multiphoton microscopy of whole mouse organs is possible by optically clearing the organ before imaging, but not all protocols preserve the fluorescent signal of fluorescent proteins. Using an optical clearing method with ethanol-based dehydration and benzyl alcohol:benzyl benzoate clearing, we show high-resolution multiphoton images of whole mouse brain expressing YFP.
07:04
Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
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Cited by 2 •
2019
We describe an experimental procedure for measuring neuronal activity through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1-GCaMP6s transgenic mice using 2-photon (2P) microscopy in vivo.
08:47
Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration
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Cited by 12 •
2012
We have developed a novel technique of quantifying nicotinic acetylcholine receptor changes within subcellular regions of specific subtypes of CNS neurons to better understand the mechanisms of nicotine addiction by using a combination of approaches including fluorescent protein tagging of the receptor using the knock-in approach and spectral confocal imaging.
04:49
Installation of Optical Windows in the Brain of Transgenic Mice to Image Neural Activities
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2025
This video demonstrates the brain's neural activity recording through an optical window in transgenic mice. The mice express a fluorescent protein that enables the detection of neural activities. A small portion of the skull is carefully removed to install an optical window, and the skull is then covered with black cement to block light before imaging.
05:10
Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
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Cited by 5 •
2010
This protocol describes the isolation and dissociation of mouse medulloblastoma tissue, and subsequent allografting of the tumor cells into immunocompromised recipient mice in order to initiate secondary medulloblastoma.
FAQs
Expression depends on the Thy1 promoter, which drives YFP production only in selected neuronal populations rather than uniformly across all neurons. This selective labeling allows investigators to follow identifiable cell bodies and processes while preserving surrounding unlabeled tissue as context. The resulting pattern supports focused analysis of neuronal architecture and connectivity in neuroscience experiments.
Researchers can examine labeled cell bodies, dendrites, axons, and synaptic projections within the same experimental model. Comparing these structures helps reveal whether an observed change affects neuronal shape, long-range processes, or synaptic organization. This broad structural coverage is useful when evaluating developmental changes, neural remodeling, or degeneration rather than focusing on a single cellular compartment.
Fluorescence microscopy makes the genetically labeled neuronal structures visible without requiring separate staining. This direct visualization simplifies observation of neuronal morphology in both living and fixed tissue. It also allows investigators to examine structural features in their tissue context, supporting comparisons of neuronal organization and changes associated with development, plasticity, injury, or disease.
Changes in the appearance or organization of labeled neuronal processes can provide measurable evidence of neural remodeling. Investigators may assess structural differences in dendrites, axons, or synaptic projections across experimental conditions, developmental stages, or disease-related states. Because the same labeled structures can be examined in living or fixed tissue, the model supports direct morphological comparisons.
A study typically uses the mice as the source of genetically labeled neuronal tissue, prepares living or fixed samples as appropriate, and examines the YFP signal with fluorescence microscopy. Investigators then compare neuronal morphology, connectivity, or structural changes between relevant experimental conditions. The workflow centers on visualizing labeled structures and interpreting differences as evidence of neural organization or remodeling.
These mice are particularly useful when a study needs to track neuronal structure during neurodevelopment, synaptic plasticity, axonal injury, or neurodegenerative disease. In each setting, fluorescence-based visualization helps investigators examine how labeled neuronal processes are organized or altered. The model therefore connects cellular morphology with broader questions about neural growth, adaptation, damage, and degeneration.
Experiments can produce visual and measurable information about neuronal morphology, connectivity, and structural change. Observed alterations in labeled cell bodies or neuronal processes may indicate neural remodeling or degeneration, depending on the research context. These outcomes help relate microscopic structural patterns to processes such as development, plasticity, axonal injury, and neurodegenerative disease.