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Establishment of a Density Gradient Centrifugation System to Isolate and Purify Bacterial Extracellular Vesicles (BEVs) from Human Faeces (Video) | JoVE

Enhanced Bacterial Extracellular Vesicles Isolation for Microbiota Multi-Omics

Article DOI: 10.3791/200625-v • 03:07 min • September 1st, 2023
September 1st, 2023 •
*1, *1, *1, 1, 1, 1, 1, 1, 1, 1
1Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University
* These authors contributed equally

Tags

Isolation And Purification Bacterial Extracellular Vesicles Human Feces Density Gradient Centrifugation Gut Microbiota Bioactive Molecules Proteins Lipids Nucleic Acids Physiology Pathology Secretion Function Optimization Top-down Centrifugation Mode Bottom-up Centrifugation Mode Particle Morphology Size Concentration Protein Content Recovery Rates Purity Levels
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